Insights from a combination of theoretical and experimental methods for probing the biomolecular interactions between human serum albumin and clomiphene.

ABSTRACT: In this study, the interaction of clomiphene (CLO), a non-steroidal and ovulatory stimulant drug employed in the treatment of infertility, with human serum albumin (HSA), the most abundant plasma transport protein, was investigated using spectrofluorometric, FT-IR, UV-Vis, and molecular modeling methods. The obtained results indicated that the binding of CLO to HSA led to intense fluorescence quenching of HSA via a static quenching mechanism, and that the process of CLO binding to HSA was enthalpy driven. By using experimental and theoretical methods, it was confirmed that as a result of binding CLO, slight conformational changes in HSA occurred. Also, the negative ΔH of interaction indicated that the binding of CLO with HSA was mainly enthalpy driven. The experimental and co

SUBMITTER: Moradi SZ 

PROVIDER: S-EPMC9091486 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature