Effect of corneal stromal lenticule customization on neurite distribution and excitatory property.

ABSTRACT:

Introduction

Refractive stromal lenticules from Small Incision Lenticule Extraction (SMILE), though usually discarded, hold a potential for various ophthalmic applications, including refractive correction, stromal volume expansion, and biomechanical strengthening of the cornea.

Objectives

To investigate the effect of lenticule customization on lenticule neurite length profile and the excitatory response (calcium signaling) and the potential of reinnervation.

Methods

Human and porcine stromal lenticules were treated by (1) excimer laser reshaping, (2) ultraviolet A-riboflavin crosslinking (CXL), and (3) decellularization by sodium dodecyl sulfate (SDS), respectively. The overall neurite scaffold immuno-positive to TuJ1 (neuron-specific class III β-tubulin) expression a

SUBMITTER: Yam GH 

PROVIDER: S-EPMC9091752 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature