An In Silico Methodology That Facilitates Decision Making in the Engineering of Nanoscale Protein Materials.

ABSTRACT: Under the need for new functional and biocompatible materials for biomedical applications, protein engineering allows the design of assemblable polypeptides, which, as convenient building blocks of supramolecular complexes, can be produced in recombinant cells by simple and scalable methodologies. However, the stability of such materials is often overlooked or disregarded, becoming a potential bottleneck in the development and viability of novel products. In this context, we propose a design strategy based on in silico tools to detect instability areas in protein materials and to facilitate the decision making in the rational mutagenesis aimed to increase their stability and solubility. As a case study, we demonstrate the potential of this methodology to improve the stability of a humanize

SUBMITTER: Parlade E 

PROVIDER: S-EPMC9099527 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature