Dataset Information

DNA methylation may affect beef tenderness through signal transduction in Bos indicus.

ABSTRACT:

Background

Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.

Results

Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different methylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.

Conclusions

We present differentially methylated loci that may be of interest to decipher the epigenetic mechanisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candidate pathway associated with beef tenderness via methylation.

SUBMITTER: de Souza MM

PROVIDER: S-EPMC9107245 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

DNA methylation may affect beef tenderness through signal transduction in Bos indicus.

de Souza Marcela Maria MM Niciura Simone Cristina Méo SCM Rocha Marina Ibelli Pereira MIP Pan Zhangyuan Z Zhou Huaijun H Bruscadin Jennifer Jessica JJ da Silva Diniz Wellison Jarles WJ Afonso Juliana J de Oliveira Priscila Silva Neubern PSN Mourão Gerson B GB Zerlotini Adhemar A Coutinho Luiz Lehmann LL Koltes James E JE de Almeida Regitano Luciana Correia LC

Epigenetics & chromatin 20220513 1

<h4>Background</h4>Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.<h4>Results</h4>Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after ...[more]

PMID: 35562812

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Dataset Information

DNA methylation may affect beef tenderness through signal transduction in Bos indicus.

Background

Results

Conclusions

Publications

DNA methylation may affect beef tenderness through signal transduction in Bos indicus.

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