Using Light-Sheet Microscopy to Study Spontaneous Activity in the Developing Lateral-Line System.

ABSTRACT: Hair cells are the sensory receptors in the auditory and vestibular systems of all vertebrates, and in the lateral-line system of aquatic vertebrates. The purpose of this work is to explore the zebrafish lateral-line system as a model to study and understand spontaneous activity in vivo. Our work applies genetically encoded calcium indicators along with light-sheet fluorescence microscopy to visualize spontaneous calcium activity in the developing lateral-line system. Consistent with our previous work, we show that spontaneous calcium activity is present in developing lateral-line hair cells. We now show that supporting cells that surround hair cells, and cholinergic efferent terminals that directly contact hair cells are also spontaneously active. Using two-color functional imaging

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC9112283 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature