Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi.

ABSTRACT:

Background

Knowledge on regulatory networks associated with cellulase biosynthesis is prerequisite for exploitation of such regulatory systems in enhancing cellulase production with low cost. The biological functions of intron retention (IR) and nonsense-mediated mRNA decay (NMD) in filamentous fungi is lack of study, let alone their roles in cellulase biosynthesis.

Results

We found that major cellulase genes (cel7a, cel7b, and cel3a) exhibited concomitant decrease in IR rates and increase in their gene expression in T. reesei under cellulase-producing condition (cellulose and lactose) that was accompanied with a more active NMD pathway, as compared to cellulase non-producing condition (glucose). In the presence of the NMD pathway inhibitor that successfully repressed the NM

SUBMITTER: Gao Y 

PROVIDER: S-EPMC9118705 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature