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Shielding of actin by the endoplasmic reticulum impacts nuclear positioning.


ABSTRACT: Nuclear position is central to cell polarization, and its disruption is associated with various pathologies. The nucleus is moved away from the leading edge of migrating cells through its connection to moving dorsal actin cables, and the absence of connections to immobile ventral stress fibers. It is unclear how these asymmetric nucleo-cytoskeleton connections are established. Here, using an in vitro wound assay, we find that remodeling of endoplasmic reticulum (ER) impacts nuclear positioning through the formation of a barrier that shields immobile ventral stress fibers. The remodeling of ER and perinuclear ER accumulation is mediated by the ER shaping protein Climp-63. Furthermore, ectopic recruitment of the ER to stress fibers restores nuclear positioning in the absence of Climp-63. Our findings suggest that the ER mediates asymmetric nucleo-cytoskeleton connections to position the nucleus.

SUBMITTER: Janota CS 

PROVIDER: S-EPMC9120458 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Shielding of actin by the endoplasmic reticulum impacts nuclear positioning.

Janota Cátia Silva CS   Pinto Andreia A   Pezzarossa Anna A   Machado Pedro P   Costa Judite J   Campinho Pedro P   Franco Cláudio A CA   Gomes Edgar R ER  

Nature communications 20220519 1


Nuclear position is central to cell polarization, and its disruption is associated with various pathologies. The nucleus is moved away from the leading edge of migrating cells through its connection to moving dorsal actin cables, and the absence of connections to immobile ventral stress fibers. It is unclear how these asymmetric nucleo-cytoskeleton connections are established. Here, using an in vitro wound assay, we find that remodeling of endoplasmic reticulum (ER) impacts nuclear positioning t  ...[more]

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