Dataset Information

Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice.

ABSTRACT:

Scope

Male fertility and sperm quality are negatively affected by psychological stress. Chronic restraint stress (CRS) is a common psychological stress that has a negative effect on sperm. Betaine (BET), an active ingredient isolated from Lycium barbarum, has anti-oxidant, anti-inflammatory and other pharmacological activities. This study aims to explore whether betaine has a therapeutic effect on sperm deformity and vitality under CRS and its mechanism.

Methods and results

Chronic restraint stress was induced in 8-week-old male C57BL/6 J mice by fixation for 6 h a day for 35 days. Mice were intraperitoneally injected with betaine (BET) or normal saline (NS) for 14 days. Thirty-five days later, the animals were sacrificed. The results showed that the detrimental effects of CRS on testes as evident by disrupted histoarchitecture, increased oxidative stress, inflammation and apoptosis that compromised male fertility. BET injections can reverse these symptoms.

Conclusions

BET can improve spermatogenesis dysfunction caused by CRS, which may provide potential dietary guidance.

SUBMITTER: Meng X

PROVIDER: S-EPMC9123792 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice.

Meng Xingqi X Peng Lixuan L Xu Jie J Guo Dongming D Cao Wenyu W Xu Yang Y Li Suyun S

Reproductive biology and endocrinology : RB&E 20220521 1

<h4>Scope</h4>Male fertility and sperm quality are negatively affected by psychological stress. Chronic restraint stress (CRS) is a common psychological stress that has a negative effect on sperm. Betaine (BET), an active ingredient isolated from Lycium barbarum, has anti-oxidant, anti-inflammatory and other pharmacological activities. This study aims to explore whether betaine has a therapeutic effect on sperm deformity and vitality under CRS and its mechanism.<h4>Methods and results</h4>Chroni ...[more]

PMID: 35597951

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Project description:Spermiogenesis is an important physiological process of mammalian fertilization. The germ cells are susceptible to the harmful effects of either psychological or physiological stress, which could induce male infertility. Our previous studies have found that chronic psychological stress could decrease sexual motivation. However, molecular mechanisms underlying male reproductive toxicity induced by chronic stress remain elusive. Recently, autophagy is proven to be involved in regulating the survival of germ cells, which is related to apoptosis. Herein, we established a chronic psychological stress model and a chronic pain model (physiological stressor) to explore the roles of autophagy in germ cells. Thirty-two male Sprague-Dawley rats were randomly divided into four groups, including the control group, the chronic psychological stress group, the SNI-sham group, and the chronic pain stress group. After exposure to stress for 35 days, open field test and the unconditioned sexual motivation test were performed. Following the behavioral experiment, autophagy in the rat testis was detected by Western blot and immunohistochemistry. We found both chronic psychological stress and chronic pain stress reduced total travel distance, the frequency of central crossing and increased the sensitivity to mechanical pain. While chronic psychological stress, but not the chronic pain stress declined sexual motivation. Chronic psychological stress prompt the expression of LC3-II with the decreased expression of p62, indicating that chronic psychological stress induced autophagy in rat testis. However, there was no significant difference between the expression of LC3-II and p62 in male rats under chronic pain stress. Therefore, chronic psychological stress and chronic pain stress have common behavior changes, but due to its unpredictability, chronic psychological stress leads to a decline in sexual motivation in male rats and induced the autophagy in testicular tissues.

Dataset Information

Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice.

Scope

Methods and results

Conclusions

Publications

Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice.

Similar Datasets

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