In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate.

ABSTRACT:

Background

Influenza A virus (IAV) infection remains a serious public health threat. Due to drug resistance and side effects of the conventional antiviral drugs, repurposing the available natural compounds with high tolerability and fewer side effects has attracted researchers' attention. The aim of this study was to screen in vitro anti-influenza activity of three anionic compounds ascorbate, acetate, and citrate.

Methods

The non-cytotoxic concentration of the compounds was determined by MTT assay and examined for the activity against IAV in simultaneous, pre-, and post-penetration combination treatments over 1 h incubation on Madin-Darby Canine Kidney (MDCK) cell line. The virus titer and viral load were determined using hemagglutination assay (HA) and qPCR, respectively.

SUBMITTER: Shokouhi Targhi H 

PROVIDER: S-EPMC9125540 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature