Dataset Information

Virological and Serological Assessment of US Army Trainees Isolated for Coronavirus Disease 2019.

ABSTRACT:

Background

Laboratory screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key mitigation measure to avoid the spread of infection among recruits starting basic combat training in a congregate setting. Because viral nucleic acid can be detected persistently after recovery, we evaluated other laboratory markers to distinguish recruits who could proceed with training from those who were infected.

Methods

Recruits isolated for coronavirus disease 2019 (COVID-19) were serially tested for SARS-CoV-2 subgenomic ribonucleic acid (sgRNA), and viral load (VL) by reverse-transcriptase polymerase chain reaction (RT-PCR), and for anti- SARS-CoV-2. Cluster and quadratic discriminant analyses of results were performed.

Results

Among 229 recruits isolated for COVID-19, those with a RT-PCR cycle threshold >30.49 (sensitivity 95%, specificity 96%) or having sgRNA log10 RNA copies/mL <3.09 (sensitivity and specificity 96%) at entry into isolation were likely SARS-CoV-2 uninfected. Viral load >4.58 log10 RNA copies/mL or anti-SARS-CoV-2 signal-to-cutoff ratio <1.38 (VL: sensitivity and specificity 93%; anti-SARS-CoV-2: sensitivity 83%, specificity 79%) had comparatively lower sensitivity and specificity when used alone for discrimination of infected from uninfected.

Conclusions

Orthogonal laboratory assays used in combination with RT-PCR may have utility in determining SARS-CoV-2 infection status for decisions regarding isolation.

SUBMITTER: Hakre S

PROVIDER: S-EPMC9129211 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Publications

Virological and Serological Assessment of US Army Trainees Isolated for Coronavirus Disease 2019.

Hakre Shilpa S Lakhal-Naouar Ines I King David B DB Burns Jennifer L JL Jackson Kenya N KN Krauss Stephen W SW Chandrasekaran Prabha P McCauley Melanie D MD Ober Shepherd Brittany L BL McHenry Samantha S Bianchi Elizabeth J EJ Ouellette Jason J Darden Janice M JM Sanborn Aaron D AD Daye Sharon P SP Kwon Paul O PO Stubbs Jeremiah J Brigantti Crystal L CL Hall Tara L TL Beagle Milford H MH Pieri Jason A JA Frambes Timothy R TR O'Connell Robert J RJ Modjarrad Kayvon K Murray Clinton K CK Jagodzinski Linda L LL Scott Paul T PT Peel Sheila A SA

The Journal of infectious diseases 20221101 10

<h4>Background</h4>Laboratory screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key mitigation measure to avoid the spread of infection among recruits starting basic combat training in a congregate setting. Because viral nucleic acid can be detected persistently after recovery, we evaluated other laboratory markers to distinguish recruits who could proceed with training from those who were infected.<h4>Methods</h4>Recruits isolated for coronavirus disease 2019 (COVI ...[more]

PMID: 35543272

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Dataset Information

Virological and Serological Assessment of US Army Trainees Isolated for Coronavirus Disease 2019.

Background

Methods

Results

Conclusions

Publications

Virological and Serological Assessment of US Army Trainees Isolated for Coronavirus Disease 2019.

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