Novel Demodex detection method involving non-invasive sebum collection and next-generation sequencing.

ABSTRACT:

Introduction

Demodex mites are common human ectoparasites found across a broad geographical range. They reside in pilosebaceous units of the skin and feed on sebum, epithelial and glandular cells. D. folliculorum is the more common mite, inhabiting the upper end of the pilosebaceous unit while D. brevis resides deeper in the skin and meibomian glands. Until now, Demodex mites have been obtained by various techniques such as skin scraping, cellophane tape, plucking eyelashes, and also by invasive biopsies.

Aim

To assess whether non-invasively collected sebum samples of patients suspected of rosacea or demodicosis are suitable for NGS DNA Demodex analysis.

Material and methods

Suspicion of seborrheic dermatitis or rosacea was the inclu

SUBMITTER: Kowalczyk MJ 

PROVIDER: S-EPMC9131945 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature