A high-throughput multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors.

ABSTRACT: Genetically encoded fluorescent biosensors are powerful tools used to track chemical processes in intact biological systems. However, the development and optimization of biosensors remains a challenging and labor-intensive process, primarily due to technical limitations of methods for screening candidate biosensors. Here we describe a screening modality that combines droplet microfluidics and automated fluorescence imaging to provide an order of magnitude increase in screening throughput. Moreover, unlike current techniques that are limited to screening for a single biosensor feature at a time (e.g. brightness), our method enables evaluation of multiple features (e.g. contrast, affinity, specificity) in parallel. Because biosensor features can covary, this capability is essential for rapid

SUBMITTER: Koveal D 

PROVIDER: S-EPMC9133083 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature