Dataset Information

Dataset of pollutants in organic and non-organic food.

ABSTRACT: Organic and non-organic equivalent corresponding type of foods were assessed for pollutants, polycyclic aromatic hydrocarbons (PAHs), pesticides and heavy metals. In total, 35 samples of sausages and cheeses were tested from three western European countries, Spain, France, and Germany. These samples were chosen because they are meat and milk products presenting some bioaccumulation capacity. Petroleum residues were demonstrated in pesticide formulants. These include polycyclic aromatic hydrocarbons (PAHs) and metals. We measured in these human foods 800 pesticides, 24 PAHs, using accredited methods, as well as eleven metals and elements. Pesticides' measurements were performed either by gas or liquid chromatography, followed by mass spectrometry, according to regulatory standard methods, that were measured over the threshold of 10 μg/kg for this reason. This could be insufficient to detect all toxic contaminations. Metals were analysed by high frequency induced plasma emission spectrometry and mass spectrometry after pressurized digestion. PAHs were assessed by gas and/or high-performance liquid chromatography with reverse phase polarity and if necessary double mass spectrometry. The raw data are summarized in Tables 1 and 2. The dataset can be reused for statistical analyses by the scientific community to evidence food pollution and to be compared with other measurements.

SUBMITTER: Seralini GE

PROVIDER: S-EPMC9133552 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Dataset of pollutants in organic and non-organic food.

Seralini Gilles-Eric GE Douzelet Jérôme J Jungers Gérald G

Data in brief 20220519

Organic and non-organic equivalent corresponding type of foods were assessed for pollutants, polycyclic aromatic hydrocarbons (PAHs), pesticides and heavy metals. In total, 35 samples of sausages and cheeses were tested from three western European countries, Spain, France, and Germany. These samples were chosen because they are meat and milk products presenting some bioaccumulation capacity. Petroleum residues were demonstrated in pesticide formulants. These include polycyclic aromatic hydrocarb ...[more]

PMID: 35647239

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Project description:Exposure to persistent organic pollutants (POPs), such as dichlorodiphenyltrichloroethane (DDT) and polychlorinated biphenyls (PCBs), has historically been linked to population collapses in wildlife. Despite international regulations, these legacy chemicals are still currently detected in women of reproductive age, and their levels correlate with reduced ovarian reserve, longer time-to-pregnancy, and higher risk of infertility. However, the specific modes of action underlying these associations remain unclear. Here, we examined the effects of five commonly occurring POPs − hexachlorobenzene (HCB), p,p'-dichlorodiphenyldichloroethylene (DDE), 2,3,3′,4,4′,5-hexachlorobiphenyl (PCB156), 2,2′,3,4,4′,5,5′-heptachlorobiphenyl (PCB180), perfluorooctane sulfonate (PFOS) − and their mixture on human ovaries in vitro. We exposed human ovarian cancer cell lines COV434, KGN, and PA1 as well as primary ovarian cells for 24 h, and ovarian tissue containing unilaminar follicles for 6 days. RNA-sequencing of samples exposed to concentrations covering epidemiologically relevant levels revealed significant gene expression changes related to central energy metabolism in the exposed cells, indicating glycolysis, oxidative phosphorylation, fatty acid metabolism, and reactive oxygen species as potential shared targets of POP exposures in ovarian cells. Alpha-enolase (ENO1), lactate dehydrogenase A (LDHA), cytochrome C oxidase subunit 4I1 (COX4I1), ATP synthase F1 subunit alpha (ATP5A), and glutathione peroxidase 4 (GPX4) were validated as targets through qPCR in additional cell culture experiments in KGN. In ovarian tissue cultures, we observed significant effects of exposure on follicle growth and atresia as well as protein expression. All POP exposures, except PCB180, decreased unilaminar follicle proportion and increased follicle atresia. Immunostaining confirmed altered expression of LDHA, ATP5A, and GPX4 in the exposed tissues. Moreover, POP exposures modified ATP production in KGN and tissue culture. In conclusion, our results demonstrate the disruption of cellular energy metabolism as a novel mode of action underlying POP-mediated interference of follicle growth in human ovaries.

Dataset Information

Dataset of pollutants in organic and non-organic food.

Publications

Dataset of pollutants in organic and non-organic food.

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