Distinct Injury Responsive Regulatory T Cells Identified by Multi-Dimensional Phenotyping.

ABSTRACT: CD4⁺ regulatory T cells (Tregs) activate and expand in response to different types of injuries, suggesting that they play a critical role in controlling the immune response to tissue and cell damage. This project used multi-dimensional profiling techniques to comprehensively characterize injury responsive Tregs in mice. We show that CD44^high Tregs expand in response to injury and were highly suppressive when compared to CD44^low Tregs. T cell receptor (TCR) repertoire analysis revealed that the CD44^high Treg population undergo TCRαβ clonal expansion as well as increased TCR CDR3 diversity. Bulk RNA sequencing and single-cell RNA sequencing with paired TCR clonotype analysis identified unique differences between CD44^high and CD44^low

SUBMITTER: Guo F 

PROVIDER: S-EPMC9135044 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature