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Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches.

ABSTRACT: The aim of this study was to investigate the phytochemical composition of dried Roselle calyx (Hibiscus sabdariffa L.) using both ethanolic and aqueous extracts. We report the antimicrobial activities against a wide range of bacteria, yeast, and fungi. The antioxidant activities were tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and 2-2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging assays. We report also for the first time the effect of the swarming motility in Pseudomonas aeruginosa PAO1. Our results showed that the tested two extracts were a rich source of phenols, flavonoids, and tannins with different degrees. Additionally, eleven phytoconstituents were identified by LC/MS technique (Hibiscus acid: 3-caffeoylquinic acid, 5-caffeoylquinic acid, 5-feruloylquinic acid, cyanidin 3-o-glucoside, myricetin, quercetin 7-o-rutinoside, quercetin 3-o-glucoside, delphinidin 3-o-sambubioside, and kaempferol 3-o-p-coumaroyl-glucoside). Also, it was shown that the calyx extract can scavenge 86% of the DPPH radical, while the rate of 53% and 23% of inhibition of the DPPH was obtained only at the concentration of 125 and 50 µg/mL, and a small inhibition was made at a concentration of 5 μg/mL. Roselle extracts inhibited the growth of the selected microorganisms at low concentrations, while higher concentrations are needed to completely kill them. However, no activity against CVB-3 was recorded for both extracts. In addition, the obtained extracts reduced the swarming motility of P. aeruginosa at 2.5 mg/ml. The docking simulation showed acceptable binding affinities (up to -9.6 kcal/mol) and interaction with key residues of 1JIJ, 2QZW, and 2UVO. The obtained results highlighted the potential use of Roselle extract as a source of phytoconstituents with promising antimicrobial, antioxidant, and anti-quorum sensing activities.

SUBMITTER: Hamrita B

PROVIDER: S-EPMC9142284 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches.

Hamrita Bechr B Emira Noumi N Papetti Adele A Badraoui Riadh R Bouslama Lamjed L Ben Tekfa Mohamed-Iheb MI Hamdi Assia A Patel Mitesh M Elasbali Abdelbaset Mohamed AM Adnan Mohd M Ashraf Syed Amir SA Snoussi Mejdi M

Evidence-based complementary and alternative medicine : eCAM 20220520

The aim of this study was to investigate the phytochemical composition of dried Roselle calyx (Hibiscus sabdariffa L.) using both ethanolic and aqueous extracts. We report the antimicrobial activities against a wide range of bacteria, yeast, and fungi. The antioxidant activities were tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and 2-2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging assays. We report also for the first time the effect of the swarming m ...[more]

PMID: 35646135

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Project description:BackgroundMultidrug-resistant (MDR) bacteria pose a significant threat to human health worldwide by increasing the harmful impact of traditional synthetic antibiotics. Traditional medicinal plants have bioactive metabolites that can significantly modulate the growth rate, cell survival, and pathogenicity of antibiotic-resistant bacteria. Hibiscus sabdariffa L., known as Roselle or Karkade, belongs to the Malvaceae family. It is well-known for its edible aromatic red/purple calyces and is extensively utilized in the food industry and pharmacological applications. H. sabdariffa calyx bioactive phytocompounds have potent therapeutic activities such as antimicrobial, antidiabetic, antiobesity, antioxidant, anti-inflammatory, and anticancer properties.MethodsThis study utilized gas chromatography-mass spectrometry (GC-MS) analysis to determine the volatile aromatic compounds that found in the hydroethanolic extract of Hibiscus sabdariffa calyces. The purpose was to verify the antibacterial properties of Roselle calyces against selective MDR clinical bacterial isolates, including A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa.ResultsThe GC-MS spectrum profile revealed the presence of twenty-seven volatile organic components, including organic fatty acid derivatives, ester compounds, sugar derivatives, and terpene components. The major GC-MS fractionations and the main active chemical compositions of the hydroethanolic extract of H. sabdariffa flowers were (E)-10-Octadecenoic acid methyl ester (59.23%), 8,11-Octadecadienoic acid, methyl ester (11.51%), Butanedioic acid, 3-hydroxy-2,2-dimethyl-, diethyl ester (6.22%), Diethyl succinate/Butanedioic acid, diethyl ester (2.35%), and Heptadecanoic acid, 16-methyl-, methyl ester/Methyl isostearate (2.31%). The hydroethanolic extract of H. sabdariffa dried calyces demonstrated potent antibacterial properties (zones diameter of inhibition growth, MIC, MBC, and MBC/MIC) against selective MDR clinical bacterial isolates, such as A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa, as determined by the phytochemical screening (TAC, TFC, and TPC) and antioxidant activity (DPPH). The surface morphological characteristics of the treated A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa clinical isolates have been affected in comparison to the untreated forms by the hydroethanolic extract of H. sabdariffa calyces, as determined by scanning electron microscopy (SEM). In silico predictive investigation revealed that the volatile aromatic components of the hydroethanolic extract of Roselle calyces exhibited significant scoring functions, binding affinities, and non-covalent intermolecular interactions with the MenB lyase and DNA gyrase targets of E. coli. These interactions significantly enhanced the activities of the volatile aromatic components against the bacterial pathogenicity, cell survival, growth, and differentiation of selective MDR clinical bacterial isolates.ConclusionsAccording to the in vitro and in silico findings, the hydroethanolic extract of H. sabdariffa calyces has shown potentials as an effective antioxidant and antibacterial treatment. It contains volatile aromatic compounds that can modulate selective MDR Gram-negative clinical bacterial isolates.

Dataset Information

Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of Hibiscus sabdariffa L. Calyx Extracts: In Vitro and In Silico Modelling Approaches.

Publications

Phytochemical Analysis, Antioxidant, Antimicrobial, and Anti-Swarming Properties of <i>Hibiscus sabdariffa</i> L. Calyx Extracts: <i>In Vitro</i> and <i>In Silico</i> Modelling Approaches.

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