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The Mycovirome in a Worldwide Collection of the Brown Rot Fungus Monilinia fructicola.


ABSTRACT: The fungus Monilinia fructicola is responsible for brown rot on stone and pome fruit and causes heavy yield losses both pre- and post-harvest. Several mycoviruses are known to infect fungal plant pathogens. In this study, a metagenomic approach was applied to obtain a comprehensive characterization of the mycovirome in a worldwide collection of 58 M. fructicola strains. Deep sequencing of double-stranded (ds)RNA extracts revealed a great abundance and variety of mycoviruses. A total of 32 phylogenetically distinct positive-sense (+) single-stranded (ss)RNA viruses were identified. They included twelve mitoviruses, one in the proposed family Splipalmiviridae, and twelve botourmiaviruses (phylum Lenarviricota), eleven of which were novel viral species; two hypoviruses, three in the proposed family Fusariviridae, and one barnavirus (phylum Pisuviricota); as well as one novel beny-like virus (phylum Kitrinoviricota), the first one identified in Ascomycetes. A partial sequence of a new putative ssDNA mycovirus related to viruses within the Parvoviridae family was detected in a M. fructicola isolate from Serbia. The availability of genomic sequences of mycoviruses will serve as a solid basis for further research aimed at deepening the knowledge on virus-host and virus-virus interactions and to explore their potential as biocontrol agents against brown rot disease.

SUBMITTER: De Miccolis Angelini RM 

PROVIDER: S-EPMC9147972 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i>.

De Miccolis Angelini Rita Milvia RM   Raguseo Celeste C   Rotolo Caterina C   Gerin Donato D   Faretra Francesco F   Pollastro Stefania S  

Journal of fungi (Basel, Switzerland) 20220506 5


The fungus <i>Monilinia fructicola</i> is responsible for brown rot on stone and pome fruit and causes heavy yield losses both pre- and post-harvest. Several mycoviruses are known to infect fungal plant pathogens. In this study, a metagenomic approach was applied to obtain a comprehensive characterization of the mycovirome in a worldwide collection of 58 <i>M. fructicola</i> strains. Deep sequencing of double-stranded (ds)RNA extracts revealed a great abundance and variety of mycoviruses. A tota  ...[more]

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