Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.).

ABSTRACT:

Background

Although the genome for the allotetraploid bioenergy crop switchgrass (Panicum virgatum) has been established, limitations in mutant resources have hampered in planta gene function studies toward crop optimization. Virus-induced gene silencing (VIGS) is a versatile technique for transient genetic studies. Here we report the implementation of foxtail mosaic virus (FoMV)-mediated gene silencing in switchgrass in above- and below-ground tissues and at different developmental stages.

Results

The study demonstrated that leaf rub-inoculation is a suitable method for systemic gene silencing in switchgrass. For all three visual marker genes, Magnesium chelatase subunit D (ChlD) and I (ChlI) as well as phytoene desaturase (PDS), phenotypic changes were observed in leaves,

SUBMITTER: Tiedge K 

PROVIDER: S-EPMC9150325 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature