Dataset Information

Greenbug (Schizaphis graminum) Herbivory Effect on Switchgrass (Panicum virgatum) PhosphoProteomeGreenbug (Schizaphis graminum) Herbivory Effect on Switchgrass (Panicum virgatum) PhosphoProteome

ABSTRACT: Cereal aphids can successfully colonize and damage switchgrass (Panicum virgatum) plants. Among the aphids tested, greenbugs (Schizaphis graminum, GB) caused significant plant damage likely through a combination of aphid-salivary proteins that are injected into plants during feeding and a strong host response elicited by herbivory. In this study, changes in protein phosphorylation present in GB-infested and uninfested control plants was determined. These data were compared against transcriptome changes recently published for this system.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Panicum Virgatum (switchgrass)

TISSUE(S): Leaf

SUBMITTER: Sophe Alvarez

LAB HEAD: Sophie Alvarez

PROVIDER: PXD018546 | Pride | 2022-02-04

REPOSITORIES: Pride

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Publications

Greenbug (Schizaphis graminum) herbivory significantly impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum).

Zogli Prince P Alvarez Sophie S Naldrett Michael J MJ Palmer Nathan A NA Koch Kyle G KG Pingault Lise L Bradshaw Jeffrey D JD Twigg Paul P Heng-Moss Tiffany M TM Louis Joe J Sarath Gautam G

Scientific reports 20200909 1

Switchgrass (Panicum virgatum L.) is an important crop for biofuel production but it also serves as host for greenbugs (Schizaphis graminum Rondani; GB). Although transcriptomic studies have been done to infer the molecular mechanisms of plant defense against GB, little is known about the effect of GB infestation on the switchgrass protein expression and phosphorylation regulation. The global response of the switchgrass cultivar Summer proteome and phosphoproteome was monitored by label-free pro ...[more]

PMID: 32908168

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Project description:In most aphid species, facultative parthenogenetic reproduction allows rapid growth and formation of large single-genotype colonies. Upon predator attack, individual aphids emit an alarm pheromone to warn the colony of this danger. (E)-beta-farnesene (EBF) is the predominant constituent of the alarm pheromone in Myzus persicae (green peach aphid) and many other aphid species. Continuous exposure to alarm pheromone in aphid colonies raised on transgenic Arabidopsis thaliana plants that produce EBF leads to habituation of the aphid population. Whereas naïve aphids are repelled by EBF, habituated aphids show no avoidance response. Individual aphids from the habituated colony can revert back to being EBF-sensitive in three generations, indicating that this behavioral change is not caused by a genetic mutation. Instead, DNA microarray experiments comparing gene expression in naïve and habituated aphids treated with EBF demonstrate an almost complete desensitization in the transcriptional response to EBF. Furthermore, EBF-responsive aphids, but not habituated aphids show significantly lower reproduction in the presence of EBF. Although both naïve and habituated aphids emit EBF upon damage, EBF-responsive aphids display a higher survival rate in the presence of coccinellid predators and thus outperform habituated aphids that do not show an avoidance response. These results provide direct evidence that aphid perception of conspecific alarm pheromone aids in predator avoidance and thereby bestows fitness benefits in survivorship and fecundity. Although habituated M. persicae have equal fecundity on control and EBF-producing plants, such transgenics may have practical applications in agriculture because of increased predation of habituated aphids. Log fold-changes (LogFC) were computed and contigs with P-values ≤ 0.05 were considered to be differentially expressed (see Supplementary file at foot of this record). Agilent 8x15K array previously reported by Ramsey et al., 2007 - BMC Genomics 8: 243. Two-condition experiment using Alexa Fluor 555 and 647 dyes. Biological replicates: 4 control replicates, 4 treated replicates.

Dataset Information

Greenbug (Schizaphis graminum) Herbivory Effect on Switchgrass (Panicum virgatum) PhosphoProteomeGreenbug (Schizaphis graminum) Herbivory Effect on Switchgrass (Panicum virgatum) PhosphoProteome

Publications

Greenbug (Schizaphis graminum) herbivory significantly impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum).

Similar Datasets

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