Diagnostic power of one-step and two-step RT-qPCR methods to SARS‑CoV‑2 detection.

ABSTRACT:

Background

Coronavirus-2019 (COVID-2019) is a novel coronavirus known as Acute Respiratory Syndrome (SARS-CoV-2). The premier standard test for SARS-CoV-2 diagnosis is a one-step RT-qPCR method, which requires specific probes and reagents. Therefore, detection on a large scale is expensive and cannot be very accurate.

Methods

A cost-effective technique based on SYBR green was evaluated in the current study. The specific primers for S and N genes were designed, then performed the cross-reactivity test with other coronavirus and respiratory viruses positive samples. Moreover, the analytical sensitivity test was carried out with 8 dilutions (1:10). Lastly, the SARS-CoV-2 clinical samples (n = 210) were tested by these two methods, and receiver operating characteristic (ROC) ana

SUBMITTER: Malekshahi A 

PROVIDER: S-EPMC9152310 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature