Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi.

ABSTRACT: An important step in the proteomic analysis of missing proteins is the use of a wide range of tissues, optimal extraction, and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. This work describes a purification protocol for identifying low-abundance proteins in human chorionic villi using the proposed "1DE-gel concentration" method. This involves the removal of SDS in a short electrophoresis run in a stacking gel without protein separation. Following the in-gel digestion of the obtained holistic single protein band, we used the peptide mixture for further LC-MS/MS analysis. Statistically significant results were derived from six datasets, containing three treatments, each from two tissue sources (elective or missed abortions). T

SUBMITTER: Shkrigunov T 

PROVIDER: S-EPMC9164042 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature