Inducible nitric oxide synthase regulates macrophage polarization via the MAPK signals in concanavalin A-induced hepatitis.

ABSTRACT:

Introduction

Acute liver inflammatory reactions contribute to many health problems; thus, it is critical to understand the underlying pathogenic mechanisms of acute hepatitis. In this study, an experimental in vivo model of concanavalin A (ConA)-induced hepatitis was used.

Materials and methods

C57BL/6 (wild-type, WT) or inducible nitric oxide synthase-deficient (iNOS^{- /-} ) mice were injected with PBS or 15 mg/kg ConA via tail vein. Detection of liver injury by histological examination and apoptosis, and flow cytometry to detect the effect of immune cells on liver injury.

Results

iNOS^{- /-}  mice had lower levels of the liver enzymes aspartate aminotransferase and alanine aminotransferase, suggesting that they were protected a

SUBMITTER: Yao X 

PROVIDER: S-EPMC9168548 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature