Single-cell-resolution map of human retinal pigment epithelium helps discover subpopulations with differential disease sensitivity.

ABSTRACT: Regional phenotypic and functional differences in the retinal pigment epithelium (RPE) monolayer have been suggested to account for regional susceptibility in ocular diseases such as age-related macular degeneration (AMD), late-onset retinal degeneration (L-ORD), and choroideremia (CHM). However, a comprehensive description of human topographical RPE diversity is not yet available, thus limiting the understanding of regional RPE diversity and degenerative disease sensitivity in the eye. To develop a complete morphometric RPE map of the human eye, artificial intelligence–based software was trained to recognize, segment, and analyze RPE borders. Five statistically different, concentric RPE subpopulations (P1 to P5) were identified using cell area as a parameter, including a subpopulation (P4

SUBMITTER: Ortolan D 

PROVIDER: S-EPMC9171647 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature