A Nonconventional Archaeal Fluorinase Identified by In Silico Mining for Enhanced Fluorine Biocatalysis.
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ABSTRACT:
SUBMITTER: Pardo I
PROVIDER: S-EPMC9173684 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
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A Nonconventional Archaeal Fluorinase Identified by In Silico Mining for Enhanced Fluorine Biocatalysis.
Pardo Isabel I Bednar David D Calero Patricia P Volke Daniel C DC Damborský Jiří J Nikel Pablo I PI
ACS catalysis 20220519 11
Fluorinases, the only enzymes known to catalyze the transfer of fluorine to an organic molecule, are essential catalysts for the biological synthesis of valuable organofluorines. However, the few fluorinases identified so far have low turnover rates that hamper biotechnological applications. Here, we isolated and characterized putative fluorinases retrieved from systematic in silico mining and identified a nonconventional archaeal enzyme from <i>Methanosaeta</i> sp. that mediates the fastest S<s ...[more]
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