Structural insights into human brain-gut peptide cholecystokinin receptors.

ABSTRACT: The intestinal hormone and neuromodulator cholecystokinin (CCK) receptors CCK1R and CCK2R act as a signaling hub in brain-gut axis, mediating digestion, emotion, and memory regulation. CCK receptors exhibit distinct preferences for ligands in different posttranslational modification (PTM) states. CCK1R couples to G_s and G_q, whereas CCK2R primarily couples to G_q. Here we report the cryo-electron microscopy (cryo-EM) structures of CCK1R-G_s signaling complexes liganded either by sulfated cholecystokinin octapeptide (CCK-8) or a CCK1R-selective small-molecule SR146131, and CCK2R-G_q complexes stabilized by either sulfated CCK-8 or a CCK2R-selective ligand gastrin-17. Our structures reveal a location-conserved yet charge-distinct pocket disc

SUBMITTER: Ding Y 

PROVIDER: S-EPMC9174195 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature