Crystal structure and metal binding properties of the periplasmic iron component EfeM from Pseudomonas syringae EfeUOB/M iron-transport system.

ABSTRACT: EfeUOB/M has been characterised in Pseudomonas syringae pathovar. syringae as a novel type of ferrous-iron transporter, consisting of an inner-membrane protein (EfeU_Psy) and three periplasmic proteins (EfeO_Psy, EfeM_Psy and EfeB_Psy). The role of an iron permease and peroxidase function has been identified for the EfeU and EfeB proteins, respectively, but the role of EfeO/M remains unclear. EfeM_Psy is an 'M75-only' EfeO-like protein with a C-terminal peptidase-M75 domain (EfeO_II/EfeM family). Herein, we report the 1.6 Å resolution crystal structure of EfeM_Psy, the first structural report for an EfeM component of P. syringae pv. syringae. The structure possesses the bi-lobate architecture found in other bacterial per

SUBMITTER: Rajasekaran MB 

PROVIDER: S-EPMC9174327 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature