Rapid Screening of Lipase Inhibitors in Scutellaria baicalensis by Using Porcine Pancreatic Lipase Immobilized on Magnetic Core-Shell Metal-Organic Frameworks.

ABSTRACT: As for ligand fishing, the current immobilization approaches have some potential drawbacks such as the small protein loading capacity and difficult recycle process. The core-shell metal-organic frameworks composite (Fe₃O₄-COOH@UiO-66-NH₂), which exhibited both magnetic characteristics and large specific surface area, was herein fabricated and used as magnetic support for the covalent immobilization of porcine pancreatic lipase (PPL). The resultant composite Fe₃O₄-COOH@UiO-66-NH₂@PPL manifested a high loading capacity (247.8 mg/g) and relative activity recovery (101.5%). In addition, PPL exhibited enhanced tolerance to temperature and pH after immobilization. Then, the composite Fe₃O₄-COOH@UiO-66-NH

SUBMITTER: Xu J 

PROVIDER: S-EPMC9182405 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature