Kinetochore tracking in 3D from lattice light-sheet imaging data with KiT.

ABSTRACT:

Motivation

Lattice light-sheet microscopy (LLSM) is revolutionizing cell biology since it enables fast, high-resolution extended imaging in three dimensions combined with a drastic reduction in photo-toxicity and bleaching. However, analysis of such datasets still remains a major challenge.

Results

Automated tracking of kinetochores, the protein complex facilitating and controlling microtubule attachment of the chromosomes within the mitotic spindle, provides quantitative assessment of chromosome dynamics in mitosis. Here, we extend existing open-source kinetochore tracking software (KiT) to track (and pair) kinetochores throughout prometaphase to anaphase in LLSM data. One of the key improvements is a regularization term in the objective function to enforce biological infor

SUBMITTER: Harrison JU 

PROVIDER: S-EPMC9191203 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature