Siglec-F-expressing neutrophils are essential for creating a profibrotic microenvironment in renal fibrosis.

ABSTRACT: The roles of neutrophils in renal inflammation are currently unclear. On examining these cells in the unilateral ureteral obstruction murine model of chronic kidney disease, we found that the injured kidney bore a large and rapidly expanding population of neutrophils that expressed the eosinophil marker Siglec-F. We first verified that these cells were neutrophils. Siglec-F+ neutrophils were recently detected in several studies in other disease contexts. We then showed that a) these cells were derived from conventional neutrophils in the renal vasculature by TGF-β1 and GM-CSF; b) they differed from their parent cells by more frequent hypersegmentation, higher expression of profibrotic inflammatory cytokines, and notably, expression of collagen 1; and c) their depletion reduced collagen dep

SUBMITTER: Ryu S 

PROVIDER: S-EPMC9197522 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature