Morphological pseudotime ordering and fate mapping reveal diversification of cerebellar inhibitory interneurons.

ABSTRACT: Understanding how diverse neurons are assembled into circuits requires a framework for describing cell types and their developmental trajectories. Here we combine genetic fate-mapping, pseudotemporal profiling of morphogenesis, and dual morphology and RNA labeling to resolve the diversification of mouse cerebellar inhibitory interneurons. Molecular layer interneurons (MLIs) derive from a common progenitor population but comprise diverse dendritic-, somatic-, and axon initial segment-targeting interneurons. Using quantitative morphology from 79 mature MLIs, we identify two discrete morphological types and presence of extensive within-class heterogeneity. Pseudotime trajectory inference using 732 developmental morphologies indicate the emergence of distinct MLI types during migration, before

SUBMITTER: Wang WX 

PROVIDER: S-EPMC9197879 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature