On-chip microfluidic buffer swap of biological samples in-line with downstream dielectrophoresis.

ABSTRACT: Microfluidic cell enrichment by dielectrophoresis, based on biophysical and electrophysiology phenotypes, requires that cells be resuspended from their physiological media into a lower conductivity buffer for enhancing force fields and enabling the dielectric contrast needed for separation. To ensure that sensitive cells are not subject to centrifugation for resuspension and spend minimal time outside of their culture media, we present an on-chip microfluidic strategy for swapping cells into media tailored for dielectrophoresis. This strategy transfers cells from physiological media into a 100-fold lower conductivity media by using tangential flows of low media conductivity at 200-fold higher flow rate versus sample flow to promote ion diffusion over the length of a straight channel archit

SUBMITTER: Huang X 

PROVIDER: S-EPMC9203925 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature