A Chemical Mutagenesis Approach to Insert Post-translational Modifications in Aggregation-Prone Proteins.

ABSTRACT: Neurodegenerative diseases are a class of disorders linked to the formation in the nervous system of fibrillar protein aggregates called amyloids. This aggregation process is affected by a variety of post-translational modifications, whose specific mechanisms are not fully understood yet. Emerging chemical mutagenesis technology is currently striving to address the challenge of introducing protein post-translational modifications, while maintaining the stability and solubility of the proteins during the modification reaction. Several amyloidogenic proteins are highly aggregation-prone, and current modification procedures can lead to unexpected precipitation of these proteins, affecting their yield and downstream characterization. Here, we present a method for maintaining amyloidogenic prot

SUBMITTER: Ge Y 

PROVIDER: S-EPMC9204764 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature