Performance of Idylla^™ RAS-BRAF mutation test for formalin-fixed paraffin-embedded tissues of colorectal cancer.

ABSTRACT:

Background

The Biocartis Idylla^™ platform is a fully automated, real-time PCR-based diagnostic system. The Idylla^™ KRAS and NRAS-BRAF Mutation Tests have been developed for the qualitative detection of mutations in KRAS, NRAS and BRAF genes, facilitating the genomic profiling of patients with colorectal cancer. The aim of the present study was to evaluate clinical performances of these tests in Japan.

Methods

The RAS and BRAF mutation statuses of 253 formalin-fixed paraffin-embedded (FFPE) colorectal cancer tissues were analyzed using the Investigational Use Only Idylla^™ KRAS Mutation Test and the Idylla^™ NRAS-BRAF Mutation Test and an in vitro diagnostics (IVD) kit (MEBGEN RASKET^™-B kit).

Results

The success rate

SUBMITTER: Makutani Y 

PROVIDER: S-EPMC9209352 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature