The Alteration Profiles of m⁶A-Tagged circRNAs in the Peri-Infarct Cortex After Cerebral Ischemia in Mice.

ABSTRACT: The N6-methyladenosine (m⁶A) modification acts as a dynamic regulatory factor in diseases by regulating the metabolism and function of the transcriptome, especially mRNAs. However, little is known regarding the functional effects of m⁶A modifications on circRNAs. In this research, we established a distal middle cerebral artery occlusion (MCAO) model in adult C57BL/6J mice. The mice were divided into three groups: sham surgery, 3 days after MCAO (3d), and 7 days after MCAO (7d). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) demonstrated that the mRNA expression levels of m⁶A-related methyltransferases (METTL3, METTL14), demethylases (FTO, ALKBH5), and reading proteins (YTHDF1, YTHDF3) altered compared to the sham group. Furthermore,

SUBMITTER: Li Y 

PROVIDER: S-EPMC9209773 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature