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A Distinct Dexamethasone-Dependent Gene Expression Profile in the Lungs of COVID-19 Patients.

ABSTRACT: The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19-associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (with or without DXM) and 8 non-COVID-19 critically ill controls. CARDS with DXM was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, phagocytosis, and FC-γ receptor signaling. Most interferon-stimulated genes were upregulated in CARDS, particularly in CARDS without DXM. In conclusion, DXM treatment was not associated with regulation of proinflammatory pathways in CARDS but with regulation of other local immune responses. Clinical Trials Registration. NCT04354584.

SUBMITTER: Fahnoe U

PROVIDER: S-EPMC9213855 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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A Distinct Dexamethasone-Dependent Gene Expression Profile in the Lungs of COVID-19 Patients.

Fahnøe Ulrik U Ronit Andreas A Berg Ronan M G RMG Jørgensen Sofie E SE Mogensen Trine H TH Underwood Alexander P AP Scheel Troels K H TKH Bukh Jens J Plovsing Ronni R RR

The Journal of infectious diseases 20221201 12

The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19-associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (with or without DXM) and 8 non-COVID-19 critically ill controls. CARDS with DXM was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, ...[more]

PMID: 35639922

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