OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels.

ABSTRACT: Interactions between physical forces and membrane proteins underpin many forms of environmental sensation and acclimation. Microbes survive osmotic stresses with the help of mechanically gated ion channels and osmolyte transporters. Plant mechanosensitive ion channels have been shown to function in defense signaling. Here, we engineered genetically encoded osmolality sensors (OzTracs) by fusing fluorescent protein spectral variants to the mechanosensitive ion channels MscL from E. coli or MSL10 from A. thaliana. When expressed in yeast cells, the OzTrac sensors reported osmolality changes as a proportional change in the emission ratio of the two fluorescent protein domains. Live-cell imaging revealed an accumulation of fluorescent sensors in internal aggregates, presumably de

SUBMITTER: Kleist TJ 

PROVIDER: S-EPMC9221499 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature