The Essential and the Nonessential Roles of Four Clock Elements in the Circadian Rhythm of Metarhiziumrobertsii.

ABSTRACT: FRQ (frequency protein), FRH (FRQ-interacting RNA helicase), and WC1 and WC2 (white collar proteins) are major clock elements that govern the circadian rhythm in Neurosporacrassa. However, deletion of frh is lethal for the viability of N. crassa, making it elusive whether FRH is essential or nonessential for the circadian rhythm. This needs clarification in a fungus where frh deletion is not lethal. Here, the nuclear FRH ortholog proved nonessential for the circadian rhythm of Metarhiziumrobertsii. The nucleocytoplasmic shuttling of M. robertsii FRQ, WC1, and WC2 orthologs was light-dependent. Yeast two-hybrid assay validated interactions of FRQ with FRH and WC1 instead of FRH with WC1 and WC2 or FRQ with WC2. The circadian rhythm wel

SUBMITTER: Peng H 

PROVIDER: S-EPMC9224670 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature