Metabolic basis for the evolution of a common pathogenic Pseudomonas aeruginosa variant.

ABSTRACT: Microbes frequently evolve in reproducible ways. Here, we show that differences in specific metabolic regulation rather than inter-strain interactions explain the frequent presence of lasR loss-of-function (LOF) mutations in the bacterial pathogen Pseudomonas aeruginosa. While LasR contributes to virulence through its role in quorum sensing, lasR mutants have been associated with more severe disease. A model based on the intrinsic growth kinetics for a wild type strain and its LasR^- derivative, in combination with an experimental evolution based genetic screen and further genetics analyses, indicated that differences in metabolism were sufficient to explain the rise of these common mutant types. The evolution of LasR^- lineages in laboratory and cl

SUBMITTER: Mould DL 

PROVIDER: S-EPMC9224983 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature