An intein-split transactivator for intersectional neural imaging and optogenetic manipulation.

ABSTRACT: The cell-type-specific recording and manipulation is instrumental to disentangle causal neural mechanisms in physiology and behavior and increasingly requires intersectional control; however, current approaches are largely limited by the number of intersectional features, incompatibility of common effectors and insufficient gene expression. Here, we utilized the protein-splicing technique mediated by intervening sequences (intein) and devised an intein-based intersectional synthesis of transactivator (IBIST) to selectively control gene expression of common effectors in multiple-feature defined cell types in mice. We validated the specificity and sufficiency of IBIST to control fluorophores, optogenetic opsins and Ca²⁺ indicators in various intersectional conditions. The IBIST-ba

SUBMITTER: Chen HS 

PROVIDER: S-EPMC9226064 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature