MHBSt¹⁶⁷ induced autophagy promote cell proliferation and EMT by activating the immune response in L02 cells.

ABSTRACT:

Background

Hepatitis B virus can induce hepatocellular carcinoma (HCC) by inducing a host immune response against infected hepatocytes. C-terminally truncated middle surface protein (MHBSt) has been reported to contribute to HCC through transcriptional activation in epidemiology studies, while the underlying mechanism of MHBSt-induced HCC is unknown.

Methods

In this study, a premature stop at codon 167 in MHBS (MHBSt¹⁶⁷) was investigated into eukaryotic expression plasmid pcDNA3.1(-). MHBSt¹⁶⁷ expressed plasmid was transfected into the L02 cell line, cell proliferation was analyzed by CCK-8 and high-content screening assays, the cell cycle was analyzed by flow cytometry, and epithelial-to-mesenchymal transition and autophagy were analyzed by immunoblott

SUBMITTER: Cheng B 

PROVIDER: S-EPMC9235077 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature