Effects of compressive stress combined with mechanical vibration on osteoclastogenesis in RAW 264.7 cells.

ABSTRACT:

Objectives

To investigate the effects of compressive force and/or mechanical vibration on NFATc1, DCSTAMP, and CTSK (cathepsin K) gene expression and the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells in RAW 264.7 cells, a murine osteoclastic-like cell line.

Materials and methods

RAW 264.7 cells were subjected to mechanical vibration, compressive force, or compressive force combined with vibration. Cell viability and the numbers of TRAP-positive multinucleated cells were evaluated. NFATc1, DCSTAMP, and CTSK gene expressions were analyzed using real-time quantitative reverse transcription polymerase chain reaction.

Results

Compressive force combined with mechanical vibration significantly increased the numbers of TRAP-positive multin

SUBMITTER: Changkhaokham B 

PROVIDER: S-EPMC9235393 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature