Glutamine deprivation impairs function of infiltrating CD8⁺ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis.

ABSTRACT:

Background

The functions of infiltrating CD8⁺ T cells are often impaired due to tumor cells causing nutrient deprivation in the tumor microenvironment. Thus, the mechanisms of CD8⁺ T cell dysfunction have become a hot research topic, and there is increased interest on how changes in metabolomics correlate with CD8⁺ T cell dysfunction.

Aim

To investigate whether and how glutamine metabolism affects the function of infiltrating CD8⁺ T cells in hepatocellular carcinoma.

Methods

Immunohistochemical staining and immunofluorescence were performed on surgically resected liver tissues from patients. Differentially expressed genes in infiltrating CD8⁺ T cells in hepatocellular carcinoma were detected using RNA sequencing.

SUBMITTER: Wang W 

PROVIDER: S-EPMC9244988 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature