Systematic identification of molecular mediators of interspecies sensing in a community of two frequently coinfecting bacterial pathogens.

ABSTRACT: Bacteria typically exist in dynamic, multispecies communities where polymicrobial interactions influence fitness. Elucidating the molecular mechanisms underlying these interactions is critical for understanding and modulating bacterial behavior in natural environments. While bacterial responses to foreign species are frequently characterized at the molecular and phenotypic level, the exogenous molecules that elicit these responses are understudied. Here, we outline a systematic strategy based on transcriptomics combined with genetic and biochemical screens of promoter-reporters to identify the molecules from one species that are sensed by another. We utilized this method to study interactions between the pathogens Pseudomonas aeruginosa and Staphylococcus aureus that are frequently found i

SUBMITTER: Zarrella TM 

PROVIDER: S-EPMC9249247 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature