Strain-boosted hyperoxic graphene oxide efficiently loading and improving performances of microcystinase.

ABSTRACT: Harmful Microcystis blooms (HMBs) and microcystins (MCs) that are produced by Microcystis seriously threaten water ecosystems and human health. This study demonstrates an eco-friendly strategy for simultaneous removal of MCs and HMBs by adopting unique hyperoxic graphene oxides (HGOs) as carrier and pure microcystinase A (PMlrA) as connecting bridge to form stable HGOs@MlrA composite. After oxidation, HGOs yield inherent structural strain effects for boosting the immobilization of MlrA by material characterization and density functional theory calculations. HGO₅ exhibits higher loading capacities for crude MlrA (1,559 mg·g^-1) and pure MlrA (1,659 mg·g^-1). Moreover, the performances of HGO₅@MlrA composite, including the capability of removing MCs and HMBs, the ecological and human safety compared to MlrA or HGO₅ treatment alone, have been studied. These results indicate that HGO₅ can be used as a promising candidate material to effectively improve the application potential of MlrA in the simultaneous removal of MCs and HMBs.

SUBMITTER: Liu HL 

PROVIDER: S-EPMC9250033 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature