High abundance of CDC45 inhibits cell proliferation through elevation of HSPA6.

ABSTRACT:

Objectives

CDC45 is the core component of CMG (CDC45-MCMs-GINS) complex that plays important role in the initial step of DNA replication in eukaryotic cells. The expression level of cdc45 is under the critical control for the accurate cell cycle progression. Loss-of-function of cdc45 has been demonstrated to inhibit cell proliferation and leads to cell death due to the inhibition of DNA replication and G1-phase arrest. An increasing of CDC45 inhibits cell proliferation as well. Nevertheless, a systematic analysis of the effect of high dose of CDC45 on cell physiology and behaviors is unclear. In the present study, we aimed to investigate the effects and mechanisms of high dose of CDC45 on cell behaviors.

Materials and methods

We overexpressed cdc45 in cultured cell lines, Ci

SUBMITTER: Fu Y 

PROVIDER: S-EPMC9251052 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature