Multi-Modal Single-Cell Sequencing Identifies Cellular Immunophenotypes Associated With Juvenile Dermatomyositis Disease Activity.

ABSTRACT: Juvenile dermatomyositis (JDM) is a rare autoimmune condition with insufficient biomarkers and treatments, in part, due to incomplete knowledge of the cell types mediating disease. We investigated immunophenotypes and cell-specific genes associated with disease activity using multiplexed RNA and protein single-cell sequencing applied to PBMCs from 4 treatment-naïve JDM (TN-JDM) subjects at baseline, 2, 4, and 6 months post-treatment and 4 subjects with inactive disease on treatment. Analysis of 55,564 cells revealed separate clustering of TN-JDM cells within monocyte, NK, CD8⁺ effector T and naïve B populations. The proportion of CD16⁺ monocytes was reduced in TN-JDM, and naïve B cells and CD4⁺ Tregs were expanded. Cell-type differential gene expression ana

SUBMITTER: Neely J 

PROVIDER: S-EPMC9254730 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature