Human carbamylome description identifies carbamylated α2-macroglobulin and hemopexin as two novel autoantigens in early rheumatoid arthritis.

ABSTRACT:

Objectives

Anti-carbamylated protein antibodies (anti-CarPAs) are present in RA sera and have been associated with erosive disease. The exact targets of anti-CarPAs in vivo are currently not well known; we used a proteomic approach on serum and SF of RA patients to assess the human carbamylome and to identify carbamylated autoantigens as potential biomarkers in early RA.

Methods

Mass spectrometry was performed on SF and serum from RA patients. Carbamylated proteins present in both sample types were selected as candidate autoantigens for the establishment of ELISAs. A cohort of early RA patients was tested for positivity for specific anti-CarPAs.

Results

Eleven novel carbamylated proteins were identified, and five were selected as potential autoantigens for detection o

SUBMITTER: Sidiras P 

PROVIDER: S-EPMC9258537 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature