Compatibility rules of human enhancer and promoter sequences.

ABSTRACT: Gene regulation in the human genome is controlled by distal enhancers that activate specific nearby promoters¹. A proposed model for this specificity is that promoters have sequence-encoded preferences for certain enhancers, for example, mediated by interacting sets of transcription factors or cofactors². This 'biochemical compatibility' model has been supported by observations at individual human promoters and by genome-wide measurements in Drosophila^3-9. However, the degree to which human enhancers and promoters are intrinsically compatible has not yet been systematically measured, and how their activities combine to control RNA expression remains unclear. Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active

SUBMITTER: Bergman DT 

PROVIDER: S-EPMC9262863 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature