TAX and HBZ: hFc Ɣ 1 proteins as targets for passive immunotherapy.

ABSTRACT:

Objectives

Human T leukemia virus type one (HTLV-1) causes two life-threatening diseases in around five percent of infected subjects, a T cell malignancy and a neurodegenerative disease. TAX and HBZ are the main virulence agents implicated in the manifestation of HTLV-1-associated diseases. Therefore, this study aims to produce these HTLV-1 factors as recombinant Fc fusion proteins to study the structures, their immunogenic properties as vaccines, and their capability to produce specific neutralization antibodies.

Materials and methods

TAX and HBZ sequences were chosen from the NCBI-nucleotide database, then designed as human Fc chimers and cloned into Pichia pastoris. Produced proteins were purified by HiTrap affinity chromatography and subcutaneously injected into r

SUBMITTER: Akbarin MM 

PROVIDER: S-EPMC9282740 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature