Transcriptional analysis identifies potential novel biomarkers associated with successful ex-vivo perfusion of human donor lungs.

ABSTRACT:

Background

Transplantation is an effective treatment for end-stage lung disease, but the donor organ shortage is a major problem. Ex-vivo lung perfusion (EVLP) of extended criteria organs enables functional assessment to facilitate clinical decision-making around utilization, but the molecular processes occurring during EVLP, and how they differ between more or less viable lungs, remain to be determined.

Methods

We used RNA sequencing of lung tissue to delineate changes in gene expression occurring in 10 donor lungs undergoing EVLP and compare lungs that were deemed non-transplantable (n = 4) to those deemed transplantable (n = 6) following perfusion.

Results

We found that lungs deemed unsuitable for transplantation had increased induction of innate immune pathways an

SUBMITTER: Ferdinand JR 

PROVIDER: S-EPMC9285052 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature