Enhanced phosphate absorption in intestinal epithelial cell-specific NHE3 knockout mice.

ABSTRACT:

Aims

The kidneys play a major role in maintaining P_i homeostasis. Patients in later stages of CKD develop hyperphosphatemia. One novel treatment option is tenapanor, an intestinal-specific NHE3 inhibitor. To gain mechanistic insight into the role of intestinal NHE3 in P_i homeostasis, we studied tamoxifen-inducible intestinal epithelial cell-specific NHE3 knockout (NHE3^IEC-KO ) mice.

Methods

Mice underwent dietary P_i challenges, and hormones as well as urinary/plasma P_i were determined. Intestinal ³³ P uptake studies were conducted in vivo to compare the effects of tenapanor and NHE3^IEC-KO . Ex vivo P_i transport was measured in everted gut sacs and brush border membrane vesicles. Intestina

SUBMITTER: Xue J 

PROVIDER: S-EPMC9286053 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature